Datasheet

ATMIN Antibody
CATALOG NUMBER: 7777

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 17-002 - A-431 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 73, 79, 91 kDa

Observed: 72 kDa
SPECIFICITY:ATMIN antibody is human, mouse and rat reactive. At least three isoforms of ATMIN are known to exist.
IMMUNOGEN:ATMIN antibody was raised against a 19 amino acid peptide near the amino terminus of human ATMIN.

The immunogen is located within amino acids 200 - 250 of ATMIN.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ATMIN antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ATMIN Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ATMIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ATMIN Antibody: ASCIZ, ZNF822, KIAA0431, ATM interactor, Zinc finger protein 822, ASCIZ
ACCESSION NO.:NP_056066
PROTEIN GI NO.:54792092
OFFICIAL SYMBOL:ATMIN
GENE ID:23300

Background

BACKGROUND:The ATM/ATR-substrate CHK2-interacting zinc finger protein (ATMIN), also known as ASCIZ, forms DNA damage-induced nuclear foci that contain the DNA repair protein Rad51 (1). ATMIN is also thought to be involved in embryonic development, as an absence of ATMIN causes late-embryonic lethality in mice with a range of organ development defects (2). It also activates the transcription DYNLL1, a light chain of the dynein motor complex and sequence-specific regulator of protein dimerization of numerous targets. DYNLL1 can bind to and inhibit the transcription activation domain of ATMIN, forming a simple dynamic feedback loop for DYNLL1 expression (3).
REFERENCES: 1) McNees CJ, Conlan LA, Tenis N, et al. ASCIZ regulates lesion-specific Rad51 focus formation and apoptosis after methylating DNA damage. EMBO J. 2005; 24:2447-57.
2) Jurado S, Smyth I, van Denderen B, et al. Dual functions of ASCIZ in the DNA base damage response and pulmonary organogenesis. PLoS Genet. 2010; 6:e1001170.
3) Jurado S, Conlan LA, Baker EK, et al. ATM substrate Chk2-interating Zn2+ finger (ASCIZ) is a bi-functional transcriptional activator and feedback sensor in the regulation of Dynein Light Chain (DYNLL1) expression. J. Biol. Chem. 2012; 287:3156-64.

For Research Use Only