Datasheet

ATG9B Antibody
CATALOG NUMBER: 5797

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:ATG9B antibody can be used for detection of ATG9B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
IMMUNOGEN:ATG9B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ATG9B.

The immunogen is located within amino acids 850 - 900 of ATG9B.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ATG9B Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ATG9B Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ATG9B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ATG9B Antibody: SONE, APG9L2, NOS3AS, Autophagy-related protein 9B, APG9-like 2, Protein sONE
ACCESSION NO.:NP_775952
PROTEIN GI NO.:239582720
OFFICIAL SYMBOL:ATG9B
GENE ID:285973

Background

BACKGROUND:ATG9B Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.
REFERENCES: 1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.
2) Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
3) Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.
4) Yamada Y, Suzuki NN, Hanada T, et al. The crystal structure of Atg3, an autophagy-related ubiquitin carrier protein (E2) enzyme that mediates Atg8 lipidation. J. Biol. Chem.2007; 282:8036-43.

For Research Use Only