Datasheet

ATG9A Antibody
CATALOG NUMBER: 6013

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:ATG9A antibody can be used for detection of ATG9A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1401 - Mouse Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
IMMUNOGEN:ATG9A antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ATG9A.

The immunogen is located within amino acids 720 - 770 of ATG9A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ATG9A Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ATG9A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ATG9A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ATG9A Antibody: mATG9, APG9L1, MGD3208, Autophagy-related protein 9A, APG9-like 1
ACCESSION NO.:NP_076990
PROTEIN GI NO.:116089287
OFFICIAL SYMBOL:ATG9A
GENE ID:79065

Background

BACKGROUND:ATG9A Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9A, a multi-spanning membrane protein localizing to the Golgi apparatus and late endosomes, has been proposed to mediate membrane transport to generate autophagosomes. ATG9A has also been implicated as a regulator of STING (stimulator of interferon genes)-mediated innate immune response.
REFERENCES: 1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.
2) Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
3) Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.
4) Webber JL, Young AR, and Tooze SA. Atg9 trafficking in mammalian cells. Autophagy2007; 3:54-6.

For Research Use Only