Datasheet

ASAH2 Antibody
CATALOG NUMBER: 4743

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:ASAH2 antibody can be used for detection of ASAH2 by Western blot at 1 and 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
IMMUNOGEN:ASAH2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2.

The immunogen is located within the last 50 amino acids of ASAH2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ASAH2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ASAH2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ASAH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ASAH2 Antibody: HNAC1, BCDase, LCDase, NCDase, N-CDase, HNAC1, Neutral ceramidase, Acylsphingosine deacylase 2
ACCESSION NO.:NP_063946
PROTEIN GI NO.:221218981
OFFICIAL SYMBOL:ASAH2
GENE ID:56624

Background

BACKGROUND:ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.
REFERENCES: 1) Nilsson A and Duan RD. Alkaline sphingomyelinases and ceramidases of the gastrointestinal tract. Chem. Phys. Lipids1999; 102:97-105.
2) Tani M, Okino N, Mitsutake S, et al. Molecular Cloning of the Full-length cDNA Encoding Mouse Neutral Ceramidase. J. Biol. Chem.2000; 275:11229-34.
3) Tani M, Iida H, and Ito M. O-Glycosylation of Mucin-like Domain Retains the Neutral Ceramidase on the Plasma Membranes as a Type II Integral Membrane Protein. J. Biol. Chem.2003; 278:10523-30.
4) Osawa Y, Uchinami H, Bielawski J, et al. Roles for C16-ceramide and sphingosine 1-phosphate in regulating hepatocyte apoptosis in response to tumor necrosis factor-alpha. J. Biol. Chem.2005; 280:27879-87.

For Research Use Only