Datasheet

ARTS Antibody
CATALOG NUMBER: 3025

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:ARTS antibody can be used for detection of ARTS by Western blot at 2 μg/mL. A band at 32 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1302 - Human Lung Tissue Lysate
 2) Cat. No. 1306 - Human Spleen Tissue Lysate
 3) Cat. No. 1305 - Human Kidney Tissue Lysate
 4) Cat. No. 10-101 - Human Lung Tissue Slide
PREDICTED MOLECULAR WEIGHT:32 kDa
IMMUNOGEN:ARTS antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human ARTS.

The immunogen is located within the first 50 amino acids of ARTS.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ARTS Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ARTS Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ARTS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ARTS Antibody: H5, ARTS, MART, SEP4, CE5B3, PNUTL2, hucep-7, BRADEION, hCDCREL-2, Septin-4, Apoptosis-related protein in the TGF-beta signaling pathway
ACCESSION NO.:AAG45673
PROTEIN GI NO.:12024871
OFFICIAL SYMBOL:SEPT4
GENE ID:5414

Background

BACKGROUND:ARTS Antibody: Apoptosis is related to many diseases and development. Mitochondrial proteins, such as cytochrome c, Apaf-1, and AIF play important role in apoptosis. A novel mitochondrial septin-like protein was identified recently and designated ARTS for apoptosis related protein in TGF-beta signaling pathway. ARTS that is encoded by the human septin H5/PNUTL2/CDCrel2b gene is located to mitochondria and translocates to the nucleus when apoptosis occurs. ARTS is expressed in many tissues. It enhances cell death induced by TGF-beta and, to a lesser extent, by other apoptotic agents, such as TNF-α and Fas ligand.
REFERENCES: 1) Larisch S, Yi Y, Lotan R, Kerner H, et al. A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif. Nat Cell Biol 2000;2(12):915-21
2) Depraetere V. The ARTS of apoptosis. Nat Cell Biol. 2000;2(12):E219.
3) Paavola,P., Horelli-Kuitunen,N., Palotie,A. and Peltonen,L. Characterization of a novel gene, PNUTL2, on human chromosome 17q22-q23 and its exclusion as the Meckel syndrome gene. Genomics 1999;55 (1):122-125
4) Zieger,B., Tran,H., Hainmann, I. , Wunderle,D., Zgaga-Griesz,A., Blaser,S. and Ware,J. Characterization and expression analysis of two human septin genes, PNUTL1 and PNUTL2. Gene 2000;261(2):197-203

For Research Use Only