Datasheet

ARSB Antibody
CATALOG NUMBER: 7465

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:ARSB antibody can be used for detection of ARSB by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1412 - Mouse Lung Tissue Lysate
 2) Cat. No. 1412 - Mouse Cerebellum Tissue Lysate
 3) Cat. No. 10-101 - Human Lung Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 59 kDa

Observed: 57kDa
SPECIFICITY:ARSB antibody is human, mouse and rat reactive. At least two isoforms of ARSB are known to exist; this antibody only recognizes the longest isoform.
IMMUNOGEN:ARSB antibody was raised against a 16 amino acid peptide near the carboxy terminus of human ARSB.

The immunogen is located within amino acids 460 - 510 of ARSB .
HOST SPECIES:Rabbit

Properties

PURIFICATION:ARSB Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ARSB Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ARSB antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ARSB Antibody : ASB, G4S, MPS6, Arylsulfatase B, N-acetylgalactosamine-4-sulfatase, ASB
ACCESSION NO.:NP_000037
PROTEIN GI NO.:38569405
OFFICIAL SYMBOL:ARSB
GENE ID:411

Background

BACKGROUND:Aryl sulfatase B (ARSB) forms a homodimer that hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate (1). ARSB localizes to the lysozyme as well as to the extracellular matrix (2). Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B (3), and can be treated with exogenous ARSB (4).
REFERENCES: 1) Wasserman SI and Austen KF. Arylsulfatase B of human lung. Isolation, characterization, and interaction with slow-reacting substance of anaphylaxis. J. Clin. Invest. 1976; 57:738-44.
2) Mitsunaga-Nakatsubo K, Kusunoki S, Kawakami H, et al. Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian livers. Med. Mol. Morphol. 2009; 42:63-9.
3) Valayannopoulos V, Nicely H, Harmatz P, et al. Mucopolysaccharidosis VI. Orphanet. J. Rare Dis. 2010; 5:5.
4) Harmatz P, Whitley CB, Waber L, et al. Enzyme replacement therapy in mucopolysaccharidosis VI (Maroteaux-Lamy syndrome). J. Pediatr. 2004; 144:574-80.

For Research Use Only