Datasheet

ARID1A Antibody
CATALOG NUMBER: 8173

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 133, 227, 251 kDa

Observed: 130, 260 kDa
SPECIFICITY:ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.
IMMUNOGEN:ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A.

The immunogen is located within amino acids 580 - 630 of ARID1A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ARID1A antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ARID1A antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ARID1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:AT rich interactive domain 1A (SWI-like), RP1-50O24.1, B120, BAF250, BAF250a, BM029, C1orf4, ELD, hELD, MRD14, OSA1, hOSA1, P270, SMARCF1
ACCESSION NO.:NP_006006
PROTEIN GI NO.:21264565
OFFICIAL SYMBOL:ARID1A
GENE ID:8289

Background

BACKGROUND:The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).
REFERENCES: 1) Martens JA and Winston F. Recent advances in understanding chromatin remodeling by Swi/Snf complexes. Curr. Opin. Genet. Dev. 2003; 13:136-42.
2) Nie Z, Xue Y, Yang D, et al. A specificity and targeting subunit of a human SWI/SNF family-related chromatin-remodeling complex. Mol. Cell. Biol. 2000; 20:8879-88.

For Research Use Only