Datasheet

ARF-BP1 Antibody
CATALOG NUMBER: 4213

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1224 - Daudi Cell Lysate
IMMUNOGEN:ARF-BP1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ARF-BP1.

The immunogen is located within amino acids 4250 - 4300 of ARF-BP1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ARF-BP1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ARF-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ARF-BP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ARF-BP1 Antibody: MULE, Ib772, LASU1, UREB1, HECTH9, URE-B1, ARF-BP1, HSPC272, KIAA0312, KIAA1578, E3 ubiquitin-protein ligase HUWE1, ARF-binding protein 1
ACCESSION NO.:NP_113584
PROTEIN GI NO.:61676188
OFFICIAL SYMBOL:HUWE1
GENE ID:10075

Background

BACKGROUND:ARF-BP1 Antibody: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
REFERENCES: 1) Gallagher SJ, Kefford RF and Rizos H. The ARF tumour suppressor. Int. J. Biochem. Cell Biol.2006; 38:1637-41.
2) Pomerantz J, Schreiber-Agus N, Leigeois NJ, et al. The Ink4a tumor suppressor gene product, p19ARF interacts with MDM2 and neutralizes MDM2’s inhibition of p53. Cell1998; 92:725-34.
3) Chen D, Kon N, Li M, et al. ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell2005; 121:1071-83.
4) Zhong Q, Gao W, Du F, et al. Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell2005; 121:1085-95.

For Research Use Only