Datasheet

ARC Antibody
CATALOG NUMBER: 2185

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:ARC antibody can be used for detection of ARC by Western blot at 1:500 dilution. An approximately 25 kDa band can be detected.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT:25 kDa
IMMUNOGEN:ARC antibody was raised against a 17 amino acid peptide near the amino terminus of human ARC.

The immunogen is located within the first 50 amino acids of ARC.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ARC Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ARC Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:ARC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ARC Antibody: ARC, FCM, MYP, NOP, NOP30, ARC, Nucleolar protein 3, Apoptosis repressor with CARD, Myp
ACCESSION NO.:NP_003937
PROTEIN GI NO.:4505419
OFFICIAL SYMBOL:NOL3
GENE ID:8996

Background

BACKGROUND:ARC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK BCL10, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1, and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.
REFERENCES: 1) Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases. Proc Natl Acad Sci USA 1998;95:5156-60
2) Stoss O, Schwaiger FW, Cooper TA, Stamm S. Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c. J Biol Chem 1999;274(16):10951-62
3) Geertman R, McMahon A, Sabban EL. Cloning and characterization of cDNAs for novel proteins with glutamic acid-proline dipeptide tandem repeats. Biochim Biophys Acta 1996;1306(2-3):147-52 (WD 0300)

For Research Use Only