Datasheet

ARC Antibody
CATALOG NUMBER: 2081

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Specifications
Properties
Additional Info
Background

Specifications

APPLICATIONS:ARC antibody can be used for detection of ARC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 23 kDa

Observed: 25 kDa
IMMUNOGEN:ARC antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ARC.

The immunogen is located within the last 50 amino acids of ARC.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ARC Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ARC Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ARC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ARC Antibody: ARC, FCM, MYP, NOP, NOP30, ARC, Nucleolar protein 3, Apoptosis repressor with CARD, Myp
ACCESSION NO.:NP_003937
PROTEIN GI NO.:4505419
OFFICIAL SYMBOL:NOL3
GENE ID:8996

Background

BACKGROUND:ARC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.
REFERENCES: 1) Koseki T, Inohara N, Chen S, et al. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases. Proc. Natl. Acad. Sci. USA 1998; 95:5156-60.

For Research Use Only