Datasheet

APRIL Antibody
CATALOG NUMBER: 2415

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:APRIL antibody can be used for detection of APRIL by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 1312 - Human Prostate Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 27 kDa

Observed: 27 kDa
IMMUNOGEN:APRIL antibody was raised against a synthetic peptide corresponding to amino acids within a extracellular domain of human APRIL.

The immunogen is located within amino acids 180 - 230 of APRIL.
HOST SPECIES:Rabbit

Properties

PURIFICATION:APRIL Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:APRIL Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:APRIL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:APRIL Antibody: APRIL, CD256, TALL2, ZTNF2, TALL-2, TRDL-1, UNQ383/PRO715, APRIL, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand
ACCESSION NO.:NP_003799
PROTEIN GI NO.:4507599
OFFICIAL SYMBOL:TNFSF13
GENE ID:8741

Background

BACKGROUND:APRIL Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for a proliferation-inducing ligand), TALL-2 (for TNF- and ApoL-related Leukocyte-expressed Ligand 2), and TRDL-1α (for TNF related death ligand 1α) in human and mouse. Two receptors for APRIL were recently identified and designated TACI and BCMA. APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-κB, and induces cell death. APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer.
REFERENCES: 1) Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J. Exp. Med. 1998; 188:1185-90.
2) Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J. Leukoc. Biol. 1999; 65:680-3.
3) Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 2000; 60:1021-7.
4) Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat. Immunol. 2000; 1:252-6.

For Research Use Only