Datasheet

APP Antibody
CATALOG NUMBER: 2136

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:APP antibody can be used for detection of APP and the C99 fragment by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 83 kDa

Observed: 110 kDa 
IMMUNOGEN:APP antibody was raised against a 15 amino acid peptide near the carboxy terminus of human APP.

The immunogen is located within the last 50 amino acids of APP.
HOST SPECIES:Rabbit

Properties

PURIFICATION:APP Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:APP Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:APP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, PN-II, CTFgamma, A4, Amyloid beta A4 protein, APP
ACCESSION NO.:CAA30050
PROTEIN GI NO.:28721
OFFICIAL SYMBOL:APP
GENE ID:351

Background

BACKGROUND:APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease.
REFERENCES: 1) Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988;331:525-7
2) Selkoe D.J. Cell biology of the amyloid β-protein precursor and the mechanism of Alzheimer's disease.Annu Rev Cell Biol 1994;10:373-403 (RD1299)

For Research Use Only