Datasheet

APOBEC3G Antibody
CATALOG NUMBER: 3257

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1223 - Caco-2 Cell Lysate
 2) Cat. No. 1306 - Human Spleen Tissue Lysate
 3) Cat. No. 10-901 - Human Spleen Tissue Slide
SPECIFICITY:APOBEC3G antibody will also detect the APOBEC3F isoform.
IMMUNOGEN:APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G.

The immunogen is located within the first 50 amino acids of APOBEC3G.
HOST SPECIES:Rabbit

Properties

PURIFICATION:APOBEC3G Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:APOBEC3G Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:APOBEC3G antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:APOBEC3G Antibody: A3G, ARCD, ARP9, ARP-9, CEM15, CEM-15, MDS019, bK150C2.7, dJ494G10.1APOBEC-related cytidine deaminase, APOBEC-related protein
ACCESSION NO.:NP_068594
PROTEIN GI NO.:13399304
OFFICIAL SYMBOL:APOBEC3G
GENE ID:60489

Background

BACKGROUND:APOBEC3G Antibody: The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5α which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.
REFERENCES: 1) Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96.
2) Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
3) Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15.
4) Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.

For Research Use Only