Datasheet

ApoA4 Antibody
CATALOG NUMBER: 5929

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Chicken, Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (93%), Rat: (79%), Mouse: (79%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:ApoA4 antibody can be used for detection of ApoA4 by Western blot at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 41 kDa

Observed: 40 kDa
IMMUNOGEN:ApoA4 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ApoA4.

The immunogen is located within amino acids 320 - 370 of ApoA4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ApoA4 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ApoA4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ApoA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ApoA4 Antibody: Apolipoprotein A-IV, Apolipoprotein A4, Apo-AIV
ACCESSION NO.:NCBI accession No. AAA51744
PROTEIN GI NO.:71773110
OFFICIAL SYMBOL:APOA4
GENE ID:337

Background

BACKGROUND:ApoA4 Antibody: Apolipoprotein A4 (also known as ApoA-IV) is a plasma protein that is an O-linked glycoprotein after proteolytic processing. ApoA4 is a major constituent of HDL and it also can be found on the surface of newly synthesized chylomicrons. The physiological function of ApoA4 is not completely identified so far, but evidence from several in vitro studies indicate that this apolipoprotein is involved in several steps of the reverse cholesterol transport pathway, which transports cholesterol from peripheral cells back to the liver.
REFERENCES: 1) Karathanasis SK, Oettgen P, Haddad IA, et al. Structure, evolution, and polymorphisms of the human apolipoprotein A4 gene (APOA4). Proc. Natl. Acad. Sci. USA 1986; 83: 8457-61
2) Kamboh MI, Friedlaender JS, Ahn YI, et al. A common deletion polymorphism in the apolipoprotein A4 gene and its significance in lipid metabolism. Arterioscler. Thromb. 1994; 14: 656-62
3) Kronenberg F, Stuhlinger M, Trenkwalder E, et al. Low apolipoprotein A-IV plasma concentrations in men with coronary artery disease. J. Am. Coll. Cardiol. 2000; 36:751-7.
4) Von Eckardstein A, Huang Y, Wu S, et al. Lipoproteins containing apolipoprotein A-IV but not apolipoprotein A-I take up and esterify cell-derived cholesterol in plasma. Arterioscler. Thromb. Vasc. Biol. 1995; 15:1755-63.

For Research Use Only