Datasheet

ApoA1 Antibody
CATALOG NUMBER: 5097

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (83%), Bovine: (78%), Chicken: (71%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:ApoA1 antibody can be used for detection of ApoA1 by Western blot at 1 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1304 - Human Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
IMMUNOGEN:ApoA1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ApoA1.

The immunogen is located within the first 50 amino acids of ApoA1.
HOST SPECIES:Chicken

Properties

PURIFICATION:ApoA1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ApoA1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ApoA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ApoA1 Antibody: Apolipoprotein A-I, Apolipoprotein A1, Apo-AI
ACCESSION NO.:P02647
PROTEIN GI NO.:113992
OFFICIAL SYMBOL:APOA1
GENE ID:335

Background

BACKGROUND:ApoA1 Antibody: Apolipoprotein A1 (ApoA1) is the major protein component of high density lipoprotein (HDL) in plasma. ApoA1 is synthesized in the liver and small intestine and promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT), the enzyme responsible for the formation of most plasma cholesteryl esters. Defects in ApoA1 are associated with HDL deficiency, Tangier disease, and systemic non-neuropathic amyloidosis.
REFERENCES: 1) Sorci-Thomas MG, Prack MM, Dashti N, et al. Differential effects of dietary fat on the tissue-specific expression of the apolipoprotein A-I gene: relationship to plasma concentration of high density lipoproteins. J. Lipid Res.1989; 30:1397-403.
2) Lai C-Q, Parnell LD, and Ordovas JM. The APOA1/C3/A4/A5 gene cluster, lipid metabolism and cardiovascular disease risk. Curr. Opin. Lipid.2005; 16:153-66.

For Research Use Only