Datasheet

APC3 Antibody
CATALOG NUMBER: 5723

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:APC3 antibody can be used for detection of APC3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1404 - Mouse Liver Tissue Lysate
IMMUNOGEN:APC3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human APC3.

The immunogen is located within the last 50 amino acids of APC3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:APC3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:APC3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:APC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:APC3 Antibody: APC3, HNUC, NUC2, ANAPC3, CDC27Hs, D0S1430E, D17S978E, Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 3, APC3
ACCESSION NO.:NP_001247
PROTEIN GI NO.:167466175
OFFICIAL SYMBOL:CDC27
GENE ID:996

Background

BACKGROUND:APC3 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC3, also known as CDC27, a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), is a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. APC3 contains a tetratricopeptide repeat (TPR) region and interacts with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1.
REFERENCES: 1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
2) Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.

For Research Use Only