Datasheet

APC13 Antibody
CATALOG NUMBER: 5739

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:APC13 antibody can be used for detection of APC13 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 17-005 - Jurkat Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 15 kDa

Observed: 14 kDa
IMMUNOGEN:APC13 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC13.

The immunogen is located within the last 50 amino acids of APC13.
HOST SPECIES:Rabbit

Properties

PURIFICATION:APC13 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:APC13 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:APC13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:APC13 Antibody: SWM1, APC13, Anaphase-promoting complex subunit 13, Cyclosome subunit 13
ACCESSION NO.:NP_056206
PROTEIN GI NO.:32698710
OFFICIAL SYMBOL:ANAPC13
GENE ID:25847

Background

BACKGROUND:APC13 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC13 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC13 promotes the stable association of APC3/Cdc27 and APC6/Cdc16 with the APC/C.
REFERENCES: 1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
2) Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
3) Schwickart M, Havlis J, Habermann B, et al. Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27. Mol. Cell. Biol.2004; 24:3562-76.

For Research Use Only