Datasheet

APC11 Antibody
CATALOG NUMBER: 5737

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:APC11 antibody can be used for detection of APC11 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-201 - 3T3/BALB Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 22 kDa

Observed: 22 kDa
IMMUNOGEN:APC11 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC11.

The immunogen is located within amino acids 70 - 120 of APC11.
HOST SPECIES:Rabbit

Properties

PURIFICATION:APC11 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:APC11 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:APC11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:APC11 Antibody: APC11, Apc11p, HSPC214, Anaphase-promoting complex subunit 11, Cyclosome subunit 11, APC11
ACCESSION NO.:NP_001002244
PROTEIN GI NO.:50409810
OFFICIAL SYMBOL:ANAPC11
GENE ID:51529

Background

BACKGROUND:APC11 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC11 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC11, together with APC2 and Ubc4, form the minimal APC ubiquitin ligase module.
REFERENCES: 1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
2) Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
3) Tan Z, Li B, Bharadwaj R, et al. APC Cullin protein and APC RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex. Mol. Biol. Cell2001; 12:3839-51.

For Research Use Only