Datasheet

APC10 Antibody
CATALOG NUMBER: 5735

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:APC10 antibody can be used for detection of APC10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1401 - Mouse Heart Tissue Lysate
IMMUNOGEN:APC10 antibody was raised against a 16 amino acid synthetic peptide near the center of human APC10.

The immunogen is located within amino acids 60 - 110 of APC10.
HOST SPECIES:Rabbit

Properties

PURIFICATION:APC10 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:APC10 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:APC10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:APC10 Antibody: DOC1, APC10, Anaphase-promoting complex subunit 10, Cyclosome subunit 10
ACCESSION NO.:EAX05052
PROTEIN GI NO.:119625457
OFFICIAL SYMBOL:ANAPC10
GENE ID:10393

Background

BACKGROUND:APC10 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC10 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. It has been suggested that APC10 plays a role to regulate the binding of specific substrates to the APC/C complex, similar to that of coactivators.
REFERENCES: 1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
2) Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
3) Passmore LA, McCormack EA, Au SWN, et al. Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. EMBO J.2003; 22:786-96.

For Research Use Only