Datasheet

APC1 Antibody
CATALOG NUMBER: 5717

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:APC1 antibody can be used for detection of APC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
IMMUNOGEN:APC1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human APC1.

The immunogen is located within amino acids 1810 - 1860 of APC1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:APC1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:APC1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:APC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:APC1 Antibody: APC1, MCPR, TSG24, Anaphase-promoting complex subunit 1, Cyclosome subunit 1, APC1
ACCESSION NO.:Q9H1A4
PROTEIN GI NO.:37537845
OFFICIAL SYMBOL:ANAPC1
GENE ID:64682

Background

BACKGROUND:APC1 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC1, also known as mitotic checkpoint regulator (MCPR), is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex.
REFERENCES: 1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
2) Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.

For Research Use Only