Datasheet

APBA1 Antibody
CATALOG NUMBER: 6925

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:APBA1 antibody can be used for detection of APBA1 by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 92 kDa

Observed: 92 kDa
SPECIFICITY:At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other APBA proteins.
IMMUNOGEN:APBA1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human APBA1.

The immunogen is located within amino acids 90 - 140 of APBA1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:APBA1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:APBA1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:APBA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:APBA1 Antibody: X11, X11A, LIN10, MINT1, D9S411E, X11ALPHA, X11, Amyloid beta A4 precursor protein-binding family A member 1, Adapter protein X11alpha, Mint-1
ACCESSION NO.:NP_001154
PROTEIN GI NO.:22035548
OFFICIAL SYMBOL:APBA1
GENE ID:320

Background

BACKGROUND:APBA1 Antibody: The Amyloid beta 4 (A4) precursor protein-binding family A member 1 (APBA1) is a member of the X11 protein family and a neuronal adapter protein that interacts with the Alzheimer's disease amyloid precursor protein (APP). APBA1 stabilizes APP and inhibits production of proteolytic APP fragments including the Aβ peptide that is deposited in the brains of Alzheimer's disease patients. It is thought to be involved in signal transduction processes and is a putative vesicular trafficking protein in the brain that can form a complex with the potential to couple synaptic vesicle exocytosis to neuronal cell adhesion. APBA1 can also bind to CASK, a multidomain scaffolding protein involved in brain development and synapse formation.
REFERENCES: 1) Borg JP, Ooi J, Levy E, et al. The phosphotyrosine interaction domains of X11 and FE65 bind to distinct sites on the YENPTY motif of amyloid precursor protein. Mol. Cell. Biol. 1996; 16:6229-41.
2) Mueller HT, Borg JP, Margolis B, et al. Modulation of amyloid precursor protein metabolism by X11alpha/Mint1. A deletion analysis of protein-protein interaction domains. J. Biol. Chem. 2000; 275:39302-6.
3) Maximov A, Sudhof TC, and Bezprozvanny I. Association of neuronal calcium channels with modular adaptor proteins. J. Biol. Chem. 1999; 274:24453-6
4) Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.

For Research Use Only