Datasheet

AP3M1 Antibody
CATALOG NUMBER: 6393

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (100%), Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:AP3M1 antibody can be used for detection of AP3M1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
SPECIFICITY:AP3M1 antibody may cross-react with AP3M2.
IMMUNOGEN:AP3M1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human AP3M1.

The immunogen is located within amino acids 340 - 390 of AP3M1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AP3M1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:AP3M1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AP3M1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:AP3M1 Antibody: AP-3 complex subunit mu-1, AP-3 adapter complex mu3A subunit
ACCESSION NO.:NP_036227
PROTEIN GI NO.:46370095
OFFICIAL SYMBOL:AP3M1
GENE ID:26985

Background

BACKGROUND:AP3M1 Antibody: The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3M1 is highly homologous to AP3M2 but is the preferred binding partner to the YQRL motif from the cytosolic domain of TGN38. AP3M1 has also been shown to interact with the HIV-1 virulence protein Nef.
REFERENCES: 1) Dell'Angelica EC, Ohno H, Ooi CE, et al. AP-3: an adaptor-like protein complex with ubiquitous expression. EMBO J. 1997; 16:917-28
2) Stephens DJ and Banting G. Specificity of interaction between adaptor-complex medium chains and the tyrosine-based sorting motifs of TGN38 and lgp120. Biochem. J. 1998; 335:567-72
3) Coleman SH, Van Damme N, Day JR, et al. Leucine-specific, functional interactions between human immunodeficiency virus type 1 Nef and adaptor protein complexes. J. Virol. 2005; 79:2066-78.

For Research Use Only