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Anthrax Lethal Factor Antibody
CATALOG NUMBER: 3417

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Bacteria
TESTED APPLICATIONS:ELISA
APPLICATIONS:Anthrax lethal factor antibody can be used for the detection of Anthrax LF protein in ELISA. It will detect 10 ng of free peptide at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
IMMUNOGEN:Anthrax lethal factor antibody antibody was raised against a synthetic peptide corresponding to 16 amino acids near the carboxy terminus of the Anthrax lethal factor protein.

The immunogen is located within the last 50 amino acids of Anthrax Lethal Factor.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Anthrax Lethal Factor Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Anthrax Lethal Factor Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Anthrax Lethal Factor antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Anthrax Lethal Factor Antibody: Lethal factor, Anthrax lethal toxin endopeptidase component, LF
ACCESSION NO.:P15917
PROTEIN GI NO.:50402185
OFFICIAL SYMBOL:pxo1_107
GENE ID:3361711

Background

BACKGROUND:Anthrax Lethal Factor Antibody: Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF). PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR) and capillary morphogenesis protein 2 (CMG2), although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. LF is the primary toxin of anthrax and functions as a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near their amino terminus, interfering with MAPK signaling and inducing apoptosis.
REFERENCES: 1) Schwartz MN. Recognition and management of anthrax - an update. New Engl. J. Med. 2001; 345:1621-6.
2) Moayeri M and Leppla SH. The roles of anthrax toxin in pathogenesis. Curr. Opin. Microbiol. 2004; 7:19-24.
3) Bradley KA, Mogridge J, Mourez M, et al. Identification of the cellular receptor for anthrax toxin. Nature 2001; 414:225-9.
4) Scobie HM, Rainey GJ, Bradley KA, et al. Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Proc. Natl. Acad. Sci. USA 2003; 100:5170-4.

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