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Anthrax Lethal Factor Antibody
CATALOG NUMBER: 3415

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Bacteria
TESTED APPLICATIONS:ELISA
APPLICATIONS:Anthrax lethal factor antibody can be used for the detection of Anthrax LF protein in ELISA. It will detect 10 ng of free peptide at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
IMMUNOGEN:Anthrax lethal factor antibody was raised against a synthetic peptide corresponding to 16 amino acids in the middle of the Anthrax lethal factor protein.

The immunogen is located within amino acids 420 - 470 of Anthrax Lethal Factor.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Anthrax Lethal Factor Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Anthrax Lethal Factor Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Anthrax Lethal Factor antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Anthrax Lethal Factor Antibody: Lethal factor, Anthrax lethal toxin endopeptidase component, LF
ACCESSION NO.:P15917
PROTEIN GI NO.:50402185
OFFICIAL SYMBOL:pxo1_107
GENE ID:3361711

Background

BACKGROUND:Anthrax Lethal Factor Antibody: Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF). PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR) and capillary morphogenesis protein 2 (CMG2), although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. LF is the primary toxin of anthrax and functions as a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near their amino terminus, interfering with MAPK signaling and inducing apoptosis.
REFERENCES: 1) Schwartz MN. Recognition and management of anthrax - an update. New Engl. J. Med. 2001; 345:1621-6.
2) Moayeri M and Leppla SH. The roles of anthrax toxin in pathogenesis. Curr. Opin. Microbiol. 2004; 7:19-24.
3) Bradley KA, Mogridge J, Mourez M, et al. Identification of the cellular receptor for anthrax toxin. Nature 2001; 414:225-9.
4) Scobie HM, Rainey GJ, Bradley KA, et al. Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Proc. Natl. Acad. Sci. USA 2003; 100:5170-4.

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