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Anosmin Antibody
CATALOG NUMBER: 6367

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:Anosmin antibody can be used for detection of Anosmin by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1219 - MCF7 Cell Lysate
 2) Cat. No. 17-012 - MCF7 Cell Slide
IMMUNOGEN:Anosmin antibody was raised against an 18 amino acid synthetic peptide near the center of human Anosmin.

The immunogen is located within amino acids 430 - 480 of Anosmin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Anosmin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Anosmin Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Anosmin antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Anosmin Antibody: HH1, HHA, KAL, KMS, ADMLX, WFDC19, KALIG-1, KALIG1, Anosmin-1, Adhesion molecule-like X-linked
ACCESSION NO.:NP_000207
PROTEIN GI NO.:119395746
OFFICIAL SYMBOL:KAL1
GENE ID:3730

Background

BACKGROUND:Anosmin Antibody: Mutations in Anosmin-1, an extracellular matrix-associated glycosylated protein, have been linked with Kallmann Syndrome (KS), an X-linked genetic disorder characterized by loss of smell caused by abnormal olfactory bulb development and delayed puberty caused by disrupted migration of the gonadotropin-releasing hormone neuron. Anosmin-1 has been shown to directly bind FGFR1 via its N-terminal cysteine-rich domain, whey-acidic protein-like domain, and its first FnIII repeat with the D2 and D3 ectodomains of FGFR1. It is thought that Anosmin-1 can modulate FGFR1 signaling and have opposing effects on the formation and activation of FGF2-FGFR1-heparing complex.
REFERENCES: 1) Franco B, Guioli S, Pragliola A, et al. A gene deleted in Kallmann’s syndrome shares homology with neural cell adhesion and axonal path-finding molecules. Nature 1991; 353:529-36.
2) Soussi-Yanicostas N, Hardelin JP, Arroyo-Jimenez MM, et al. Initial characterization of anosmin-1, a putative extracellular matrix protein synthesized by definite neuronal cell populations in the central nervous system. J. Cell Sci. 1996; 109:1749-57.
3) Hu Y, Guimond SE, Travers P, et al. Novel mechanisms of fibroblast growth factor receptor 1 regulation by extracellular matrix protein Anosmin-1. J. Biol. Chem. 2009; 284:29905-20

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