Datasheet

ANKLE2 Antibody
CATALOG NUMBER: 6609

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:ANKLE2 antibody can be used for detection of ANKLE2 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 121, 139, 145 kDa

Observed: 121 kDa
SPECIFICITY:Three isoforms of ANKLE2 are known to exist; this antibody will detect all three. ANKLE2 antibody is predicted to not cross-react with ANKLE1.
IMMUNOGEN:ANKLE2 antibody was raised against a 19 amino acid synthetic peptide near the center of human ANKLE2.

The immunogen is located within amino acids 280 - 330 of ANKLE2.
HOST SPECIES:Chicken

Properties

PURIFICATION:ANKLE2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ANKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ANKLE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ANKLE2 Antibody: Lem4, LEMD7, KIAA0692, LEM4, Ankyrin repeat and LEM domain-containing protein 2, LEM domain-containing protein 4
ACCESSION NO.:AAH43157
PROTEIN GI NO.:148664230
OFFICIAL SYMBOL:ANKLE2
GENE ID:23141

Background

BACKGROUND:ANKLE2 Antibody: Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. ANKLE2 (Ankyrin repeat and LEM domain containing 2) is a member of ankyrin repeat and LEM domain containing family. It is a potential single-pass membrane protein that contains three ankyrin repeats and a Lem domain. Ankyrin repeats mediate protein-protein interactions and are among the most common structural motifs in known proteins. The function of this protein is still under investigation.
REFERENCES: 1) Lee KK and Wilson KL. All in the family: evidence for four new LEM-domain proteins Lem2 (NET-25), Lem3, Lem4 and Lem5 in the human genome. Symp. Soc. Exp. Biol. 2004; 56:329-39

For Research Use Only