Datasheet

ANGPT2 Antibody
CATALOG NUMBER: 6737

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:ANGPT2 antibody can be used for detection of ANGPT2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1304 - Human Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 55 kDa

Observed: 58 kDa
SPECIFICITY:At least three isoforms of ANGPT2 are known to exist; this antibody will detect all three isoforms.
IMMUNOGEN:ANGPT2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ANGPT2.

The immunogen is located within the last 50 amino acids of ANGPT2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ANGPT2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ANGPT2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ANGPT2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ANGPT2 Antibody: ANG2, AGPT2, Angiopoietin-2, ANG-2
ACCESSION NO.:NP_001138
PROTEIN GI NO.:4557315
OFFICIAL SYMBOL:ANGPT2
GENE ID:285

Background

BACKGROUND:ANGPT2 Antibody: Angiopoietin-2 (ANGPT2) is a member of the Ang family, a family of angiogenic factors that play major roles in angiogenesis during the development and growth of human cancers, but also during lymphangiogenesis. ANGPT2 is generally considered an antagonist of ANGPT1 and endothelial TEK tyrosine kinase (TIE-2, TEK). ANGPT2 disrupts the vascular remodeling ability of ANGPT1 and is thought to induce endothelial cell apoptosis, resulting in vessel regression. Expression of ANGPT2 has been linked to invasive and metastatic phenotypes of gliomas and other cancers.
REFERENCES: 1) Hu B and Cheng SY. Angiopoetin-2: Development of inhibitors for cancer therapy. Curr. Oncol. Rep. 2009; 11:111-6.
2) Maisonpierre PC, Suri C, Jones PF, et al. Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science 1997; 277:55-60.
3) Lobov IB, Brooks PC, Lang RA, et al. Angiopoietin-2 displays VEGF-dependent modulation of capillary structure and endothelial cell survival in vivo. Proc. Natl. Acad. Sci. USA 2002; 99:11205-10.
4) Bach F, Uddin FJ, Burke D, et al. Angiopoietins in malignancy. Eur. J. Surg. Oncol. 2007; 33:7-15.

For Research Use Only