Datasheet

ANG Antibody
CATALOG NUMBER: 6983

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Horse: (75%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:ANG antibody can be used for detection of ANG by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
PREDICTED MOLECULAR WEIGHT:16 kDa
SPECIFICITY:Two alternatively spliced transcript variants have been observed.
IMMUNOGEN:ANG antibody was raised against a 15 amino acid synthetic peptide near the center of human ANG.

The immunogen is located within amino acids 70 - 120 of ANG.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ANG Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ANG Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ANG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ANG Antibody: ALS9, RAA1, HEL168, RNASE4, RNASE5, Angiogenin, Ribonuclease 5, RNase 5
ACCESSION NO.:NP_001136
PROTEIN GI NO.:4557313
OFFICIAL SYMBOL:ANG
GENE ID:283

Background

BACKGROUND:ANG Antibody: Angiogenin (ANG or ANG I) is important for the process of neovascularization and formation of new blood vessels. ANG is similar to pancreatic ribonuclease A and functions as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. It interacts with endothelial cell-surface actin and may cause changes in the cell cytoskeleton. ANG is thought to be involved in the development of solid tumors and its antagonists are capable of inhibiting tumor growth. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). Angiogenin is a genetic link between ALS and PD.
REFERENCES: 1) Moroianu J and Riordan JF. Identification of the nucleolar targeting signal of human angiogenin. Biochem. Biophys. Res. Commun. 1994; 203:1765-72.
2) Hu G, Riordan JF, and Vallee BL. Angiogenin promotes invasiveness of cultured endothelial cells by stimulation of cell-associated proteolytic activities. Proc. Natl. Acad. Sci. USA 1994; 91:12096-100.
3) Pyatibratov MG and Kostyukova AS. New insights into the role of angiogenin in actin polymerization. Int. Rev. Cell. Mol. Biol. 2012; 295:175-98.
4) Li S, Ibaragi S, and Hu GF. Angiogenin as a molecular target for the treatment of prostate cancer. Curr. Cancer Ther. Rev. 2011; 7:83-90.

For Research Use Only