Datasheet

AMIGO1 Antibody
CATALOG NUMBER: 7565

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:AMIGO1 antibody can be used for detection of AMIGO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 54 kDa

Observed: 55 kDa
SPECIFICITY:AMIGO1 antibody is human, mouse, and rat reactive. AMIGO1 antibody is predicted to not cross-react with AMIGO2 or AMIGO3.
IMMUNOGEN:AMIGO1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AMIGO1.

The immunogen is located within the last 50 amino acids of AMIGO1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AMIGO1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:AMIGO1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AMIGO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:AMIGO1 Antibody: ALI2, AMIGO, AMIGO-1, ALI2, KIAA1163, Amphoterin-induced protein 1
ACCESSION NO.:NP_065754
PROTEIN GI NO.:153791466
OFFICIAL SYMBOL:AMIGO1
GENE ID:57463

Background

BACKGROUND:The amphoterin-induced gene and ORF (AMIGO1) protein is a brain-enriched, glycosylated transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRRs) and one Ig-like domain. It and the related proteins AMIGO2 and AMIGO3 are thought to be cell adhesion molecules expressed on fiber tracts of neuronal tissues and participate in their formation (1). AMIGO1 has also been suggested to play important roles in dendritic outgrowth during development and could modulate the survival of developing and adult neurons (2).
REFERENCES: 1) Kuja-Panula J, Kiiltomaki M, Yamashiro T, et al. AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats. J. Cell Biol. 2003; 160:963-73.
2) Chen Y, Hor HH, and Tang BL. AMIGO is expressed in multiple brain cell types and may regulate dendritic growth and neuronal survival. J. Cell Physiol. 2012; 227:2217-29.

For Research Use Only