Datasheet

Alpha-tubulin Antibody
CATALOG NUMBER: 7175

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%), Chicken: (100%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:Tubulin antibody can be used for detection of Tubulin by Western blot at 0.5 and 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 50 kDa
IMMUNOGEN:Rabbit polyclonal Tubulin antibody was raised against a 16 amino acid peptide near the amino terminus of human Tubulin.

The immunogen is located within amino acids 140 - 190 of Alpha-tubulin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Alpha-tubulin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Alpha-tubulin Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Alpha-tubulin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Alpha-tubulin Antibody: TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2
ACCESSION NO.:NP_005992
PROTEIN GI NO.:17921993
OFFICIAL SYMBOL:TUBA3C
GENE ID:7278

Background

BACKGROUND:Alpha-tubulin Antibody: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.
REFERENCES: 1) McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.
2) Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53.
3) Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.

For Research Use Only