Datasheet

alpha-Tubulin Antibody [2B11]
CATALOG NUMBER: PM-7627

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Mouse, Rabbit, Rat, Zebrafish
TESTED APPLICATIONS:WB
APPLICATIONS:alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 50 kDa

Observed: 54 kDa
SPECIFICITY:alpha-Tubulin antibody is human, mouse, rat, rabbit, and zebrafish reactive.
IMMUNOGEN:alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin
HOST SPECIES:Mouse

Properties

PURIFICATION:alpha-Tubulin antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:alpha-Tubulin antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:alpha-Tubulin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Monoclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Tubulin alpha-1A, TUBA1A, TUBA3, LIS3
ACCESSION NO.:CAA25855
PROTEIN GI NO.:37492
OFFICIAL SYMBOL:TUBA1A
GENE ID:7846

Background

BACKGROUND:Alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
REFERENCES: 1) McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.
2) Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53.
3) Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.

For Research Use Only