Datasheet

Aldh3A1 Antibody
CATALOG NUMBER: 4787

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:Aldh3A1 antibody can be used for detection of Aldh3A1 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1319 - Human Stomach Tissue Lysate
SPECIFICITY:At least two isoforms of Aldh3A1 are known to exist. This antibody is predicted to have no cross-reactivity to Aldh3A2.
IMMUNOGEN:Aldh3A1 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human Aldh3A1.

The immunogen is located within the last 50 amino acids of Aldh3A1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Aldh3A1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Aldh3A1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Aldh3A1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Aldh3A1 Antibody: ALDH3, ALDHIII, ALDH3, Aldehyde dehydrogenase, dimeric NADP-preferring
ACCESSION NO.:NP_000682
PROTEIN GI NO.:22907049
OFFICIAL SYMBOL:ALDH3A1
GENE ID:218

Background

BACKGROUND:Aldh3A1 Antibody: Aldh3A1 is a member of the aldehyde dehydrogenase superfamily, a group of NAD(P)(+)-dependent enzymes that catalyze the oxidation of a wide spectrum of aliphatic and aromatic aldehydes. Aldh3A1 is highly expressed in stomach and even more strongly in cornea, representing between 5 to 50% of the water soluble protein fraction in mammalian corneas. It is thought that Aldh3A1 acts to protect the cornea from UV-induced oxidative stress by not only detoxification of reactive aldehydes by also through the direct absorbtion of UV energy. However, corneas from Aldh3A1-null mice are indistinguishable from those from wild-type mice; mice lacking both Aldh3A1 and Aldh1A1 showed increased cataract formation following UVB exposure, suggesting that Aldh1A1 may be able to compensate for the loss of Aldh3A1.
REFERENCES: 1) Vasiliou V and Pappa A. Polymorphisms of human aldehyde dehydrogenases. Consequences for drug metabolism and disease. Pharmacology2000; 61:192-8.
2) Hsu LC, Chang WC, Shibuya A, et al. Human stomach aldehyde dehydrogenase cDNA and genomic cloning, primary structure, and expression in Escheria coli. J. Biol. Chem.1992; 267:3030-7.
3) Pappa A, Sophos NA and Vasiliou V. Corneal and Stomach expression of aldehyde dehydrogenases: from fish to mammals. Chem. Biol. Interact.2001; 130:181-91.
4) Estey T, Cantore M, Weston PA, et al. Mechanisms involved in the protection of UV-induced protein inactivation by the corneal crystallin ALDH3A1. J. Biol. Chem.2007; 282:4382-92.

For Research Use Only