Datasheet

Albumin Antibody
CATALOG NUMBER: 5159

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Donkey: (85%), Horse: (85%), Pig: (85%), Rabbit: (85%), Chicken: (80%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Albumin antibody can be used for detection of Albumin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1404 - Mouse Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
 3) Cat. No. 1304 – Human Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 67 kDa

Observed: 57, 67 kDa
IMMUNOGEN:Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin.

The immunogen is located within amino acids 340 - 390 of Albumin.
HOST SPECIES:Chicken

Properties

PURIFICATION:Albumin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Albumin Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Albumin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Albumin Antibody: PRO0883, PRO0903, PRO1341, Serum albumin
ACCESSION NO.:P02768
PROTEIN GI NO.:113576
OFFICIAL SYMBOL:ALB
GENE ID:213

Background

BACKGROUND:Albumin Antibody: Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. It functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The resulting product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. At least two isoforms of Albumin are known to exist.
REFERENCES: 1) Rothschild MA, Oratz M, and Schreiber SS. Serum Albumin. Hepatology 1988; 8:385-401.

For Research Use Only