Datasheet

AKAP4 Antibody
CATALOG NUMBER: 7961

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (94%), Mouse: (88%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:AKAP4 antibody can be used for detection of AKAP4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 94 kDa

Observed: 100 kDa
SPECIFICITY:AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms.
IMMUNOGEN:AKAP4 antibody was raised against a 17 amino acid peptide near the amino terminus of human AKAP4.

The immunogen is located within the first 50 amino acids of AKAP4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AKAP4 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:AKAP4 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AKAP4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:A-kinase anchor protein 4, AKAP-4, AKAP82, hAKAP82, CT99, FSC1, HI, PRKA4
ACCESSION NO.:NP_003877
PROTEIN GI NO.:21493037
OFFICIAL SYMBOL:AKAP4
GENE ID:8852

Background

BACKGROUND:The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell (1). AKAP4, also known as hAKAP82, is a major sperm fibrous sheath protein that localizes to the entire length of the flagellum of human sperm (2). It has been suggested that AKAP4 plays multiple roles in sperm motility and regulation of signal transduction pathways (2). AKAP4 has also been postulated to be a potential biomarker for breast cancer (3).
REFERENCES: 1) Carnegie GK, Means CK, and Scott JD. A-kinase anchoring proteins: from protein complexes to physiology and disease. IUBMB 2009; 61:394-406.
2) Turner RM, Johnson LR, Haig-Ladewig L, et al. An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail. J. Biol. Chem. 1998; 273:32135-41.
3) Saini S, Jagadish N, Gupta A, et al. A novel cancer testis antigen, A-kinase anchor protein 4 (AKAP4) is a potential biomarker for breast cancer. PLoS One 2013; 8:e57095.

For Research Use Only