Datasheet

AJAP1 Antibody
CATALOG NUMBER: 8163

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:AJAP1 antibody can be used for detection of AJAP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 45 kDa

Observed: 48 kDa
SPECIFICITY:AJAP1 antibody is human, mouse and rat reactive.
IMMUNOGEN:AJAP1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human AJAP1.

The immunogen is located within amino acids 290 - 340 of AJAP1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AJAP1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:AJAP1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AJAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Adherens junctions associated protein 1, Membrane protein shrew-1, MOT8, SHREW1
ACCESSION NO.:NP_061324
PROTEIN GI NO.:9055278
OFFICIAL SYMBOL:AJAP1
GENE ID:55966

Background

BACKGROUND:The Adherens Junction Associated Protein 1 (AJAP1) plays a key role in cell adhesion and cell migration (1). It is a transmembrane protein found on the basolateral surface of epithelium and expressed by multiple types of epithelium (1,2). AJAP1 interacts with E-Cadherin and appears to promote the internalization of E-Cadherin upon growth factor stimulation (3). This facilitates the dissolution of adherens junctions with an increase in cell motility (3,4).
REFERENCES: 1) Zeng L, Fee BE, Rivas MV, et al. Adherens junctional associated protein-1: a novel 1p36 tumor suppressor candidate in gliomas (Review). Int. J. Oncol. 2014; 45:13-7.
2) Han L, Zhang KL, Zhang JX, et al. AJAP1 is dysregulated at an early stage of gliomagenesis and suppresses invasion through cytoskeleton reorganization. CNS Neurosci. Ther. 2014; 20:429-37.
3) Gross JC, Schreiner A, Engels K, et al. E-cadherin surface levels in epithelial growth factor-stimulated cells depend on adherens junction protein shrew-1. Mol. Biol. Cell 2009; 20:3598-607.
4) Lin N, Di C, Bortoff K, et al. Deletion or epigenetic silencing of AJAP1 on 1p36 in glioblastoma. Mol. Cancer Res. 2012; 10:208-17.

For Research Use Only