Datasheet

AIF Antibody
CATALOG NUMBER: 2267

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:AIF antibody can be used for detection of AIF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 1461 - Rat Heart Tissue Lysate
 3) Cat. No. 1401 - Mouse Heart Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 27, 36, 67 kDa

Observed: 71 kDa
SPECIFICITY:At least five isoforms of AIF are known to exist; this antibody will detect all isoforms except isoform 5.
IMMUNOGEN:AIF antibody was raised against a 15 amino acid peptide near the center of human AIF.

The immunogen is located within amino acids 500 - 550 of AIF.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AIF Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:AIF Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1
ACCESSION NO.:O95381
PROTEIN GI NO.:50400606
OFFICIAL SYMBOL:CNKSR1
GENE ID:10256

Background

BACKGROUND:AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.
REFERENCES: 1) Zamzami N and Kroemer G. Condensed matter in cell death. Nature 1999; 401:127-8.
2) Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999; 397:441-6.
3) Daugas E, Susin SA, Zamzami N, et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 2000; 14:729-39.

For Research Use Only