Datasheet

AIF Antibody
CATALOG NUMBER: 2239

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:AIF antibody can be used for detection of AIF by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 1205 - Jurkat Cell Lysate
 3) Cat. No. 17-005 - Jurkat Cell Slide
 4) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 67 kDa

Observed: 67 kDa
SPECIFICITY:Multiple isoforms of AIF are known to exist.
IMMUNOGEN:AIF antibody was raised against a peptide corresponding to amino acids near the amino terminus of mature human AIF.

The immunogen is located within amino acids 90 - 140 of AIF.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AIF Antibody is Ion exchange chromatography purified.
PHYSICAL STATE:Liquid
BUFFER:AIF Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1
ACCESSION NO.:O95381
PROTEIN GI NO.:50400606
OFFICIAL SYMBOL:CNKSR1
GENE ID:10256

Background

BACKGROUND:AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.
REFERENCES: 1) Zamzami N and Kroemer G. Condensed matter in cell death. Nature 1999; 401:127-8.
2) Sahara S, Aoto M, Eguchi Y, et al. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999; 401:168-73.

For Research Use Only