Datasheet

AID Antibody
CATALOG NUMBER: 3091

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:H, M
TESTED APPLICATIONS:E, WB, ICC, IF
APPLICATIONS:AID antibody can be used for detection of AID by Western blot at 2 μg/mL. Anti-AID is human and mouse reactive. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1225 - Ramos Cell Lysate
IMMUNOGEN:AID antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human AID.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AID Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:AID Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AID antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:None

Additional Info

ALTERNATE NAMES:AID Antibody: AID, ARP2, CDA2, HIGM2, HEL-S-284, AID, Single-stranded DNA cytosine deaminase, Activation-induced cytidine deaminase
ACCESSION NO.:AAM95402
PROTEIN GI NO.:22297218

Background

BACKGROUND
AID Antibody: Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.
REFERENCES
1) Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 1999; 274:18470-6.
2) Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.Cell 2000; 102:553-63.
3) Greeve J, Philipsen A, Krause K, et al. Expression of activation-induced cytidine deaminase in human B-cell Hodgkin lymphomas. Blood 2003; 101:3574-80.
4) Cascalho M. Advantages and disadvantages of cytidine deamination. J. Immunol. 2004; 172:6513-8.

For Research Use Only