Datasheet

AFP Antibody
CATALOG NUMBER: 5869

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (91%), Sheep: (83%), Bovine: (83%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:AFP antibody can be used for detection of AFP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
IMMUNOGEN:AFP antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human AFP.

The immunogen is located within amino acids 340 - 390 of AFP.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AFP Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:AFP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AFP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein
ACCESSION NO.:NP_001125
PROTEIN GI NO.:4501989
OFFICIAL SYMBOL:AFP
GENE ID:174

Background

BACKGROUND:AFP Antibody: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma. However, hereditary persistance of AFP may also be found in individuals with no obvious pathology. AFP is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. AFP levels in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
REFERENCES: 1) Lafuste P, Robert B, Mondon F, et al. Alpha-fetoprotein gene expression in early and full-term human trophoblast. Placenta2002; 23:600-12.
2) Walhof CM, Van Sonderen L, Voute PA, et al. Half-life of alpha-fetoprotein in patients with a teratoma, endodermal sinus tumor, or hepatoblastoma. Pediatr. Hematol. Oncol.1988; 5:217-27.
3) Nishio H, Heiskanen M, Palotie A, et al. Tandem arrangement of the human serum albumin multigene family in the sub-centromeric region of 4q: evolution and chromosomal direction of transcription. J. Mol. Biol.1996; 259:113-9.
4) Deutsch HF. Chemistry and biology of alpha-fetoprotein. Adv. Cancer Res.1991; 56:253-312

For Research Use Only