Datasheet

AFAP1L Antibody
CATALOG NUMBER: 6559

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (79%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1203 - A549 Cell Lysate
 2) Cat. No. 17-003 - A549 Cell Slide
SPECIFICITY:At least four isoforms of AFAP1L1 are known to exist. This antibody is predicted to not cross-react with other AFAP family members.
IMMUNOGEN:AFAP1L1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human AFAP1L1.

The immunogen is located within amino acids 70 - 120 of AFAP1L.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AFAP1L Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:AFAP1L Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AFAP1L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:AFAP1L Antibody: Actin filament-associated protein 1-like 1, AFAP1-like protein 1
ACCESSION NO.:NP_689619
PROTEIN GI NO.:55742689
OFFICIAL SYMBOL:AFAP1L1
GENE ID:134265

Background

BACKGROUND:AFAP1L Antibody: The actin filament-associated protein 1 (AFAP1)-like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is thought to have a unique function distinct from AFAP1. AFAP1L1 colocalizes with cortactin and localizes to invadosomes, highly dynamic, actin-rich adhesion structures harboring metalloproteases, suggesting that AFAP1L1 affects invadosome formation (1, 4). Recent reports suggest that AFAP1L1 can be used as a prognostic marker for spindle cell sarcomas.
REFERENCES: 1) Snyder BN, Cho Y, Qian Y, et al. AFAP1L1 is a novel adaptor protein of the AFAP family that interacts with cortactin and localizes to invadosomes. Eur. J. Cell Biol. 2011; 90:376-89
2) Flynn DC, Leu TH, Reynolds AB, et al. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament-associated pp60src substrate. Mol. Cell. Biol. 1993;13:7892-900.
3) Baisden JM, Gatesman AS, Cherezova L, et al. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases. Oncogene 2001; 20:6607-16.
4) Saltel F, Daubon T, Juin A, et al. Invadosomes: intriguing structures with promise. Eur. J. Cell Biol. 2011; 90:100-7.

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