Datasheet

ADROPIN Antibody
CATALOG NUMBER: 7527

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:ADROPIN antibody can be used for detection of ADROPIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 8 kDa

Observed: 16 kDa
SPECIFICITY:ADROPIN antibody is human, mouse and rat reactive. Despite its predicted molecular weight, ADROPIN often migrates at a higher than expected molecular weight in SDS-PAGE.
IMMUNOGEN:ADROPIN antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ADROPIN.

The immunogen is located within the last 50 amino acids of ADROPIN.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ADROPIN antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ADROPIN antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ADROPIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ADROPIN Antibody: UNQ470, C9orf165, UNQ470/PRO830, Adropin, Energy homeostasis-associated protein
ACCESSION NO.:NP_940975
PROTEIN GI NO.:58218977
OFFICIAL SYMBOL:ENHO
GENE ID:375704

Background

BACKGROUND:ADROPIN is a recently identified protein that has been implicated in the maintenance of energy homeostasis and insulin resistance (1-3). ADROPIN expression is regulated by energy status and dietary nutrient content and is altered by obesity and regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor gamma (PPAR-gamma) (1). ADROPIN levels increase with dietary fat content (2). ADROPIN has also been proposed to play a role in the regulation of endothelial function (3).
REFERENCES: 1) Kumar KG, Trevaskis JL, Lam DD, et al. Identification of Adropin as a secreted factor linking dietary macronutrient intake with energy homeostasis and lipid metabolism. Cell Metab. 2008; 8:468-81.
2) Kumar KG, Zhang J, Gao S, et al. Adropin deficiency is associated with increased adiposity and insulin resistance. Obesity 2012; 1394-402.
3) Lovren F, Pan Y, Quan A, et al. Adropin is a novel regulator of endothelial function. Circulation 2010; 122:S185-92.

For Research Use Only