Datasheet

ADRM1 Antibody
CATALOG NUMBER: 8119

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:ADRM1 antibody can be used for detection of ADRM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 45 kDa

Observed: 48 kDa
SPECIFICITY:ADRM1 antibody is human, mouse and rat reactive. At least two isoforms of ADRM1 are know to exist; this antibody will detect both isoforms.
IMMUNOGEN:ADRM1 antibody was raised against a 19 amino acid peptide near the center of human ADRM1.

The immunogen is located within amino acids 210 - 260 of ADRM1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ADRM1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ADRM1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ADRM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Adhesion regulating molecule 1, ARM1, Gp110, hRpn13, Proteasomal ubiquitin receptor ADRM1, Proteasome regulatory particle non-ATPase 13, Rpn13
ACCESSION NO.:NP_783163
PROTEIN GI NO.:28373194
OFFICIAL SYMBOL:ADRM1
GENE ID:11047

Background

BACKGROUND:The adhesion-regulating molecule 1 (ADRM1) is thought to be involved in protein recruitment and cell adhesion and functions as a proteasomal ubiquitin receptor (1). ADRM1 promotes the activity of UCH-L5 and plays a role in lymphocyte homing. The carboxy-terminal domain of mammalian ADRM1 serves to bind and enhance the isopeptidase activity of UCHL5/UCH37, perhaps serving as a mechanism to accelerate ubiquitin chain disassembly (2,3). ADRM1 expression is induced by IFN-gamma in some cancer cell lines and its expression is upregulated in other metastatic cells, suggesting a role in carcinogenesis (4,5).
REFERENCES: 1) Husnjak K, Elsasser S, Zhang N, et al. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008; 453:481-8.
2) Yao T, Song L, Xu W, et al. Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. Nat. Cell Biol. 2006; 8:994-1002.
3) Qiu XB, Ouyang SY, Li CJ, et al. hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds the deubiquitinating enzyme, UCH37. EMBO J. 2006; 25:5742-53.
4) Al-Shami A, Jhaver KG, Vogel P, et al. Regulators of the proteasome pathway, Uch37 and Rpn13, play distinct roles in mouse development. PLoS One 2010; 5:e13654.

For Research Use Only