Datasheet

Adiponectin Antibody
CATALOG NUMBER: 3551

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (71%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Adiponectin antibody can be used for the detection of adiponectin by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1209 - HL60 Cell Lysate
 2) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 27 kDa

Observed: 26 kDa
IMMUNOGEN:Adiponectin antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human adiponectin.

The immunogen is located within the last 50 amino acids of Adiponectin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Adiponectin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Adiponectin Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Adiponectin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Adiponectin Antibody: ACDC, ADPN, APM1, APM-1, GBP28, ACRP30, ADIPQTL1, ACDC, Adiponectin, 30 kDa adipocyte complement-related protein
ACCESSION NO.:NP_004788
PROTEIN GI NO.:4757760
OFFICIAL SYMBOL:ADIPOQ
GENE ID:9370

Background

BACKGROUND:Adiponectin Antibody: Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike the CTRPs, which are expressed in a wide variety of tissues, adiponectin is reported to be expressed exclusively by differentiated adipocytes. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. It is present in high levels in normal human plasma, but is reduced in obese subjects and often in those with increased insulin resistance and type 2 diabetes, suggesting that adiponectin may be a useful pharmacological target in various metabolic diseases.
REFERENCES: 1) Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.
2) Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.
3) Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
4) Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8.

For Research Use Only