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Adenovirus-9 E4 Orf1 Antibody
CATALOG NUMBER: 4749

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Virus
TESTED APPLICATIONS:ELISA
APPLICATIONS:Ad-9 E4 Orf1 antibody can detect 10ng Ad-9 E4 Orf1 peptide in ELISA at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
IMMUNOGEN:Ad-9 E4 Orf1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the Ad-9 E4 Orf1.

The immunogen is located within the last 50 amino acids of Adenovirus-9 E4 Orf1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Adenovirus-9 E4 Orf1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Adenovirus-9 E4 Orf1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Adenovirus-9 E4 Orf1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Adenovirus-9 E4 Orf1 Antibody: E4-ORF1, ORF1
ACCESSION NO.:P89079
PROTEIN GI NO.:62510674
OFFICIAL SYMBOL:E4
GENE ID:6386282

Background

BACKGROUND:Adenovirus-9 E4 Orf1 Antibody: The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents. Unlike the Ads from subgroup A and B, the ones from subgroup D, Ad9 and Ad10 elicit estrogen-dependent mammary tumors as opposed to undifferentiated sarcomas. In the case of Ad9, its tumorigenicity is dependent on the product of the open reading frame (ORF) 1 of the early region 4 (E4). The tumorigenic potential of Ad9 E4 Orf1 depends on a carboxyl-terminal PDZ domain-binding motif that mediates interactions with several different membrane-associated cellular proteins such as MUPP1, PATJ, MAGI-1, ZO-2 and Dlg1. It has been suggested that Ad9 E4 Orf1 may have evolved from an ancestral cellular dUTP pyrophosphatase.
REFERENCES: 1) Shenk T. Adenoviridae: the viruses and their replication, p. 2265-2300 in DM Knipe and PM Howley (ed.) Fields virology, 4th ed.Vol. 2. 2001. Lippincott-Raven Publishers, Philadelphia, PA.
2) Javier R, Rasha Jr. K, Macdonald GJ, et al. Human adenovirus type 9-induced rat mammary tumors. J. Virol.1991; 65:3192-202.
3) Javier RT. Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats. J. Virol.1994; 68:3917-24.
4) Weiss RS, Lee SS, Prasad BV, et al. Human adenovirus early region 4 open reading frame 1 genes encode growth-transforming proteins that may be distantly related to dUTP pyrophosphatase enzymes. 1997; J. Virol.1997; 71:1857-70.

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