Datasheet

ADAM29 Antibody
CATALOG NUMBER: 8183

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

APPLICATIONS:ADAM29 antibody can be used for detection of ADAM29 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1310 - Human Bladder Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 76 kDa

Observed: 80 kDa
SPECIFICITY:ADAM29 antibody is human specific. ADAM29 antibody is predicted to not cross-react with other ADAM family proteins.
IMMUNOGEN:ADAM29 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADAM29.

The immunogen is located within amino acids 710 - 760 of ADAM29.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ADAM29 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ADAM29 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ADAM29 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Disintegrin and metalloproteinase domain-containing protein 29, ADAM metallopeptidase domain 29, CT73, SVPH1
ACCESSION NO.:NP_055084
PROTEIN GI NO.:73765552
OFFICIAL SYMBOL:ADAM29
GENE ID:11086

Background

BACKGROUND:The ADAM (a disintegrin and metalloprotease) protein family plays a role in cell-cell and cell-matrix interface related processes, including fertilization, muscle fusion, secretion of TNFalpha (tumor necrosis factor alpha), and modulation of the neurogenic function of Notch and Delta (1-3). ADAM29 contains one disintegrin domain, one EGF-like domain and one peptidase M12B domain (2). It is highly expressed in testes (3) and may be involved in spermatogenesis and fertilization (4,5).
REFERENCES: 1) Wang F, Xu R, Zhu P, et al. Preliminarily functional analysis of a cloned novel human gene ADAM29. Sci. China C. Life Sci. 2001; 44392-9.
2) Xu R, Cai J, Xu T, et al. Molecular cloning and mapping of a novel ADAM gene (ADAM29) to human chromosome 4. Genomics 1999; 62:537-9.
3) Cerretti DP, DuBose RF, Black RA, et al. Isolation of two novel metalloproteinase-disintegrin (ADAM) cDNAs that show testis-specific gene expression. Biochem. Biophys. Res. Commun. 1999; 263:810-5.
4) Oppezzo P, Vasconcelos Y, Settegrana C, et al. The LPL/ADAM29 expression ratio is a novel prognosis indicator in chronic lymphocytic leukemia. Blood 2005; 106:650-7.

For Research Use Only