Datasheet

ACSL1 Antibody
CATALOG NUMBER: 7081

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Guinea pig: (88%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:ACSL1 antibody can be used for detection of ACSL1 by Western blot at 0.25 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1304 – Human Liver Tissue Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 41, 58, 77 kDa

Observed: 45, 47 kDa 
SPECIFICITY:At least three isoforms of ACSL1 are known to exist; this antibody will detect all three isoforms.
IMMUNOGEN:Rabbit polyclonal ACSL1 antibody was raised against an 18 amino acid peptide near the center of human ACSL1.

The immunogen is located within amino acids 240 - 290 of ACSL1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ACSL1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ACSL1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ACSL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ACSL1 Antibody: ACS1, LACS, FACL1, FACL2, LACS1, LACS2, Long-chain-fatty-acid--CoA ligase 1, Acyl-CoA synthetase 1, ACS1
ACCESSION NO.:NP_001986
PROTEIN GI NO.:40807491
OFFICIAL SYMBOL:ACSL1
GENE ID:2180

Background

BACKGROUND:ACSL1 Antibody: Long-chain acyl coenzyme A synthetase 1 (ACSL1) catalyzes the synthesis of acyl-CoA from long-chain fatty acids in an ATP-dependent manner. ACSL1 is a member of a family of long-chain acyl-CoA synthetases which differ in substrate preference, tissue expression, and subcellular localization. In mouse, ACSL1 is the major acyl-CoA enzyme in the heart, providing 60-90% of heart ATP. Loss of ACSL1 either globally or in heart ventricles resulted in impaired fatty acid oxidation, activation of the mammalian target of rapamycin (mTOR), and cardiac hypertrophy.
REFERENCES: 1) Black PN and DiRusso CC. Transmembrane movement of exogenous long-chain fatty acids: proteins, enzymes, and vectorial esterification. Microbiol. Mol. Biol. Rev. 2003; 67:454-72.
2) Coleman RA, Lewin TM, Van Horn CG, et al. Do acyl-CoA synthetases regulate fatty acid entry into synthetic versus degradative pathways? J. Nutr. 2002; 132:2123-6.
3) Clark H, Carling D, and Saggerson D. Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids. Eur. J. Biochem. 2004; 271:2215-24
4) Ellis JM, Mentock SM, DePetrillo MA, et al. Mouse cardiac acyl Coenzyme A synthetase 1 deficiency impairs fatty acid oxidation and induces cardiac hypertrophy. Mol. Cell. Biol. 2011; 31:1252-62.

For Research Use Only