Datasheet

Acinus Antibody
CATALOG NUMBER: 2217

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (100%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:Acinus antibody antibody can be used for detection of Acinus by Western blot at 0.5 μg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT:220 kDa
IMMUNOGEN:Acinus antibody was raised against a peptide corresponding to amino acids near the carbosy terminus of human AcinusL, which are identical to those of mouse Acinus.

The immunogen is located within the last 50 amino acids of Acinus.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Acinus Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Acinus Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:Acinus antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Acinus Antibody: ACN, ACINUS, fSAP152, KIAA0670, Apoptotic chromatin condensation inducer in the nucleus, Acinus
ACCESSION NO.:AAD56724
PROTEIN GI NO.:5931959
OFFICIAL SYMBOL:ACIN1
GENE ID:22985

Background

BACKGROUND:Acinus Antibody: Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS'.
REFERENCES: 1) Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
2) Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.

For Research Use Only