Datasheet

ABCB10 Antibody
CATALOG NUMBER: 45-179

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:H
TESTED APPLICATIONS:E, WB
APPLICATIONS:ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 90 kDa and 50 kDa in human bone marrow lysates after 0.05 μg/mL antibody staining.1 kDa according to NP_036221.2 and given the expected mature size of ~65 kDa according to Graf et al. (PMID: 15215243). Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IMMUNOGEN:ABCB10 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of ABCB10.
HOST SPECIES:Goat

Properties

PURIFICATION:ABCB10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
PHYSICAL STATE:Liquid
BUFFER:ABCB10 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at-20˚C. Minimize freezing and thawing.
CLONALITY:This is a polyclonal antibody.
CONJUGATE:None

Additional Info

ALTERNATE NAMES:ABCB10, ATP-binding cassette, sub-family B (MDR/TAP), member 10, EST20237, M-ABC2, MTABC2, sub-family B, ABC-me
ACCESSION NO.:NP_036221.2
PROTEIN GI NO.:171184400

Background

REFERENCES
1) Graf SA, Haigh SE, Corson ED, Shirihai OS. Targeting, import, and dimerization of a mammalian mitochondrial ATP binding cassette (ABC) transporter, ABCB10 (ABC-me). J Biol Chem. 2004 Oct 8;279(41):42954-63

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