Datasheet

ABCA7 Antibody
CATALOG NUMBER: 6889

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Specifications
Properties
Additional Info
Background

Specifications

APPLICATIONS:ABCA7 antibody can be used for detection of ABCA7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
 3) Cat. No. 17-010 - HEK293 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 236 kDa

Observed: 240 kDa
SPECIFICITY:ABCA7 antibody is predicted to not cross-react with other ABC1 subfamily proteins.
IMMUNOGEN:ABCA7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ABCA7.

The immunogen is located within amino acids 130 - 180 of ABCA7.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ABCA7 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ABCA7 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ABCA7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ABCA7 Antibody: ABCX, ABCA-SSN, ATP-binding cassette sub-family A member 7
ACCESSION NO.:NP_061985
PROTEIN GI NO.:150417984
OFFICIAL SYMBOL:ABCA7
GENE ID:10347

Background

BACKGROUND:ABCA7 Antibody: ATP-binding cassette (ABC) transporters are an evolutionarily conserved family that use ATP hydrolysis to catalyze the transport of various molecules across cell membranes. They are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCA7, a member of the ABC1 subfamily, is a full-size ABC transporter consisting of two sets of the multiple membrane-spanning domains plus the Walker motifs for the ATP interaction. ABCA7 shows the highest homology to ABCA1, an essential molecule for cholesterol homeostasis. The high expression levels of ABCA7 in peripheral leukocytes, thymus, spleen and bone marrow suggests a role in the immune system lipid homeostasis.
REFERENCES: 1) Kaminski WE, Orsó E, Diederich W, et al. Identification of a novel human sterol-sensitive ATP-binding cassette transporter (ABCA7). Biochem. Biophys. Res. Commun. 2000; 273:532-8.
2) Abe-Dohmae S, Ueda K, and Yokoyama S. ABCA7, a molecule with unknown function. FEBS Lett. 2006; 580:1178-82
3) Ikeda Y, Abe-Dohmae S, Munehira Y, et al. 2003. Post-transcriptional regulation of human ABCA7 and its function for the apoA-I-dependent lipid release. Biochem. Biophys. Res.Commun. 311:313-8.
4) Iwamoto N, Abe-Dohmae S, Sato R, et al. ABCA7 expression is regulated by cellular cholesterol through the SREBP2 pathway and associated with phagocytosis. J. Lipid Res. 2006; 47:1915-27.

For Research Use Only