YY1 Antibody | catalog# 31-219
Background
YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
Description
Western blot analysis of YY1 in Fetal Thymus Lysate using YY1 antibody at 1 μg/ml (12% gel).
Specifications
Additional Names
YY1Positive Control
- Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate]
Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by peptide affinity chromatography method.Host
YY1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human YY1.Application
YY1 antibody can be used for detection of YY1 by ELISA at 1:7812500. YY1 antibody can be used for detection of YY1 by western blot at 1.0 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WB, IHCBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store YY1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
H, M, R, DProtein Accession Number
NP_003394Molecular Weight
45 kDaReference
- Gronroos, E., et al., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12165-12170.]